Karl Fischer Reagent: A Essential Tool for Measuring Moisture Content

finar chemicals
2 min readFeb 15, 2023

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Karl Fisher Reagent, also known as Karl Fischer titration, is a widely used analytical method in laboratories across the globe. It is named after its inventor, the German chemist Karl Fischer, who developed the method in the 1930s. The reagent is used to determine the amount of water present in a sample, either as a percentage of the sample’s weight or volume.

The Karl Fisher Reagent is a mixture of iodine and sulfur dioxide in a solvent, typically methanol or ethanol. The iodine acts as an oxidizing agent, while the sulfur dioxide reduces the iodine back to its elemental form. The reaction between the iodine and water produces hydriodic acid, which can be titrated with a standardized solution of sodium thiosulfate to determine the amount of water present.

The Karl Fisher Reagent manufactured by lab chemical suppliers has a wide range of applications in the laboratory, including the determination of water content in a variety of materials such as fuels, lubricants, and pharmaceuticals. It is also commonly used in the food and beverage industry to ensure that products such as milk and fruit juice contain the appropriate amount of water.

One of the benefits of using the Karl Fisher Reagent in the laboratory is its high sensitivity and accuracy. It can detect water levels as low as one part per million, making it an ideal method for determining trace amounts of moisture. It is also a relatively quick and simple process, requiring minimal sample preparation and only a small amount of reagent.

Titration principles of Karl Fischer

I2 + 2H2O + SO2 -> 2HI + H2SO4

When the titrating agent reaches a sufficient volume to react with all of the water in the sample, the titration is considered to have reached its endpoint. This is because both water and iodine are consumed in equal amounts.

There are several reputable laboratory chemical manufacturers in India, such as Finar, that produce high-quality Karl Fisher Reagents for use in a variety of applications. These companies also offer a range of other laboratory chemicals and reagents, making them a one-stop shop for all of your lab chemical needs.

If you’re working in a laboratory, the Karl Fisher Reagent is an important tool to have in your arsenal.

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